Quantitative Comparison of Bone Marrow Aspirate Concentration By Harvest Site

There is an increasing interest in uses of bone marrow aspirate concentrate for various orthopedic procedures throughout the lower extremity. The ideal location harvest extremity surgery would be near surgical field, however, there has not been a quantitative comparison contents retrieved from locations objective this present study to assess concentration obtained sites (anterior iliac crest, proximal tibia, and calcaneus), as determined by cell surface markers osteoprogenitor cells colony forming unit assays evaluate differences growth differentiation ability. Patients that underwent involving planned use part their procedure were enrolled. three included anterior calcaneus. Samples using single retrieval system (Arthrex; Naples, FL). remaining sample was spun centrifuge develop (BMAC) BMAC sent analysis. Bone samples assessed flow cytometry progenitor cells. Specific mesenchymal stromal +CD146, +CD73, +CD271, +CD105, -CD34, -CD45. Portions then cultured with media specific adipose (CFU-Adi), osteoblast (CFU-ob), fibroblasts (CFU-F). ANOVA statistical tests used determine between groups. 14 patients enrolled (mean age 28 +/- 13.0 years) 6 4 mean percentage calcaneus 95.1%, 90.8%, 94.9%, respectively. no significant site %CD34-45- (p=0.82), %CD73+ (p=0.50), %CD105+ (p=0.09). %CD146+(p=0.51), %CD271+(p=0.70). significantly greater CFU-F (p=0.03), CFU-Ob CFU-AD (p=0.04) crest compared tibia findings suggest produce similar potential density cells, improved other sites. should during surgery. Further testing analysis CFU-chondrocytes ELISA factors cytokines are needed fully characterize ongoing.